Professor Håkan Nygren

Research Background

Professor Nygren has long term experience in work with labelled antibodies and development of immunoassays (e.g. the ELISPOT), basic histology, fluorescence microscopy, electron microscopy and has collaborated with physicists in surface science during the last 20 years. Imaging mass spectrometry was included among our analytical tools 5 years ago, and through collaboration with physicists, we have reached a world-leading position in this field of research. We were e.g. the first to publish: Analysis of organic molecules in tissue by silver coating, new techniques for tissue preparation, localization of cholesterol in the CNS, and localization of galactosylceramide in tissue. Our group has an extensive and well defined peak list of analytes.

Doctoral Thesis

PhD. Odont. , Subject area: Histology; Title of Thesis: Immunoenzyme methods; Histologiska Institutionen GU 1979-12-12.

Professor Nygrens trusty dog Fanny

PhD. Students

Jan-Henrik Elam, Blood proteins and platelets at polymer surfaces. med. dr. 23/10 1992. Opponent: Hans Elwing, IFM, LiTH.

Maria Werthén, Immunochemistry at solid-liquid interfaces. fil. dr  12/2 1993. Opponent: Ingemar Lundström, IFM, LiTH.

Simon Alaeddin, Kinetics and isotherms of protein adsorption.,  med. dr 21/3 1996. Opponent: Hans Arwin, IFM, LiTH.

Christin Karlsson, Studies on mechanisms of biocompatibility elicited at the blood material interface. med. dr 6/6 1997. Opponent: Penti Tengvall, IFM, LiTH

Sanjiv Kanagaraja, Cell and tissue reactions to metals and metal bound thiols. odont. dr 6/6 2000. Opponent: Lars Magnus Bjursten, Malmö US, LU

Cecilia Eriksson, Interaction of whole blood with TiO2 surfaces with focus on adhesion and activation of PMNLs. med. dr 15/12 2000. Opponent: Olle Stendahl. Medicinsk Mikrobiologi, Häsouniversitetet i Linköping.

Marita Broberg, Platelet reactions in the nonself recognition of material surfaces. med. dr 1/6 2001. Opponent: Tom Horbett, University of Washington, Seattle, USA.

Herman Sahlin Redox effects on Neutrophils in wound healing conducted by wound dressings and hyperbaric oxygen. medd. dr 4/4 2003. Opponent: Lars-Magnus Bjursten, Malmö.

Per Malmberg Localisation of organic ions with imaging TOF-SIMS. med. dr 25/4 2003. Opponent: Lennart Kenne, Uppsala

Invited Speaker

Gordon Conference, Biomolecular Recognition and Immobilisation, Holderness NH, Augusti 1990.

Oxygen signalling in wound healing, Columbus, Ohio september 2002.

New Biomaterials in Medicine Cardiff april 2003

Publications (2000 - )

Nygren H, Malmberg P., High resolution imaging by organic secondary ion mass spectrometry. Trends Biotechnol. 2007 Oct 4; Nygren H, Hagenhoff B, Malmberg P, Nilsson M, Richter K.
Bioimaging TOF-SIMS: High resolution 3D imaging of single cells.
Microsc Res Tech. 2007 Jul 27; [Epub ahead of print]
Eriksson C, Ohlson K, Richter K, Billerdahl N, Johansson M, Nygren H., Callus formation and remodeling at titanium implants.
J Biomed Mater Res A. 2007 Jun 21; [Epub ahead of print]
Malmberg P, Nygren H, Richter K, Chen Y, Dangardt F, Friberg P, Magnusson Y., Imaging of lipids in human adipose tissue by cluster ion TOF-SIMS. Microsc Res Tech. 2007 Sep;70(9):828-35.
Richter K, Nygren H, Malmberg P, Hagenhoff B. , Localization of fatty acids with selective chain length by imaging time-of-flight secondary ion mass spectrometry. Microsc Res Tech. 2007 Jul;70(7):640-7.
Malmberg P, Bexell U, Eriksson C, Nygren H, Richter K.
Analysis of bone minerals by time-of-flight secondary ion mass spectrometry: a comparative study using monoatomic and cluster ions sources. Rapid Commun Mass Spectrom. 2007;21(5):745-9.
Pernber Z, Richter K, Mansson JE, Nygren H. , Sulfatide with different fatty acids has unique distributions in cerebellum as imaged by time-of-flight secondary ion mass spectrometry (TOF-SIMS). Biochim Biophys Acta. 2007 Feb;1771(2):202-9. Epub 2006 Dec 22.
Malmberg P, Borner K, Chen Y, Friberg P, Hagenhoff B, Mansson JE, Nygren H. Localization of lipids in the aortic wall with imaging TOF-SIMS. Biochim Biophys Acta. 2007 Feb;1771(2):185-95. Epub 2006 Dec 19
164. C. Eriksson, K. Borner, H. Nygren, K. Ohlson, U. Bexell, N. Billerdahl, M. Johansson, Studies by imaging TOF-SIMS of bone mineralization on porous titanium implants after 1 week in bone, Applied Surface Science 252 (2006) 6757-6760.
163. K. Borner, H. Nygren, P. Malmberg, E. Tallarek, B. Hagenhoff, Localization of Na+ and K+ in rat cerebellum with imaging TOF-SIMS, Applied Surface Science 252 (2006) 6777-6781.
162. H. Nygren, K. Borner, P. Malmberg, B. Hagenhoff, Localization of cholesterol in rat cerebellum with imaging TOF-SIMS - Effect of tissue preparation, Applied Surface Science 252 (2006) 6975-6981.
161. K. Borner, H. Nygren, B. Hagenhoff, P. Malmberg, E. Tallarek, J.E. Mansson, Distribution of cholesterol and galactosylceramide in rat cerebellar white matter, Biochim Biophys Acta 1761 (2006) 335-344.
160. Nygren, H., Börner, K., Hagenhoff, B., Malmberg, P., Månsson, J-E., Localozation of cholesterol, phosphocholine and galactosylceramide in rat cerebellar cortex with imaging TOF-SIMS equipped with a bismuth cluster ion gun. Biochim. Biophys. Acta 1737(2005)102-110.
159. Nygren, H., Börner, K., Hagenhoff, B., Malmberg, P. and Tallarek, E. Imaging TOF-SIMS of Rat Kidney Prepared by High Pressure Freezing. Microscopy Research and Techniques 68(2005)329-34
158. Nygren, H. , Johansson, B. R. , Malmberg, P. Bioimaging TOF-SIMS of rat tissues by gold cluster bombardment of a silver matrix. Microscopy Research and Techniques 65 (2004) 282 - 286.
157. Nygren, H. , Malmberg P. Silver deposition on freeze dried cells allows subcellular localization of cholesterol with imaging TOF-SIMS. Journal of Microscopy, 215 (2004) 156-161.
156. Nygren H. , Malmberg, P. , Kriegskotte, C., Arlinghaus, H. F. Bioimaging TOF-SIMS: Localization of cholesterol in rat kidney sections. FEBS Letters, 566 (2004) 291-293.
155. Erikson, C. , Nygren, H. , Ohlson, K. Implantation of hydrophilic and hydrophobic titanium discs in rat tibia. Cell reactions during the first 3 weeks. Biomaterials 25 (2004) 4759-4766
154. Nygren, H. , Malmberg, P. , Sahlin, H Development of a wound dressing targetting neutrophil function. World Journal of Surgery 28 (2004) 337-342
153. Yahyapour, N., Eriksson, C., Malmberg, P., Nygren, H., Thrombin, Kallikrein, and Complement C5-9 adsorption on hydrophilic and hydrophobic titanium and glass afetr short time exposure to whole blood. Biomaterials 25 (2004) 3171-3176.
152. Sahlin, H., Nygren, H., Contact activation and down regulation of neutrophils by cellulose wound dressings can be prevented by readial scavengers. J. Biomed. Mater. Eng. 15 (2005) 13-19.
151. Malmberg, P., Nygren, H., Sjövall, P., Lausmaa, J., Subcellular Localisation of Cholesterol and Phosphocholine with Pattern-Recognition-Imaging-TOF-SIMS., Spectroscopy 18 (2004) 503 - 512
150. Sjövall, P., Lausmaa, J., Nygren, H., Carlsson, L., Malmberg, P. (2003) Imaging of membrane lipids in single cells by imprint-imaging TOF-SIMS., Anal. Chem. 75, 3429 .
149. Nygren, H., Eriksson, C., Malmberg, P., Sahlin, H., Carlsson, L., Lausmaa, J., Sjövall, P.(2003) A Cell Preparation Method Allowing Subcellular Localization of Cholesterol and Phosphocholine with Imaging TOF-SIMS. Colloids & Surfaces B: Biointerfaces, 30, 87
148. Broberg, M., Eriksson , C., Nygren, H., Öster, L. (2003) Novel in vivo method for evaluation of healing around implants in bone. J. Biomed. Mater. Res. 66, 662.
147. Broberg, M., Nygren, H. (2003) Exposure of fibrinogen-adherent platelets to plasma proteins: a new method for studying protein interactions with platelets., J Biomed Mater Res. 2003 Aug 1;66A(2):403.
146. Broberg, M., Eriksson, C., Nygren H. (2002) GPIIb/IIIa is the receptor for platelet adhesion to hydrophilic an hydrophobic model surfaces. J. Lab Clin Med 139, 163.
145. Sutherland, D., Broberg, M., Nygren, H., Kasemo, B. (2001) Influence of nanoscale surface topography and chemistry on the functional behaviour of an adsorbed model macromolecule. Macromolecular Bioscience 1, 270.
144. Yahyapour, N., Kjellstrand, P., Wieslander, A., Nygren, H., (2001) Glutathione protects neutrophils against the toxic effects of peritoneal dialysis fluid., Toxicology in vitro 15, 655.
143. Nygren, H., Hrustic, E., Karlsson, C. Öster, L. (2001) Membrane leakage and respiratory burst response of peritoneal leukocytes adhering to titanium and stainless steel. J. Biomed.Mater. Res. 57, 238
142. Malmberg, P., Nygren, H. (2001) Effect of porousity on the initial reaction between materials and whole blood. Biomaterials 23, 247.
141. Sahlin, H., Nygren, H. (2001) Cytotoxicity testing of wound-dressing materials. ATLA 29, 269.
140. Nygren, H., Broberg, M., Eriksson, C., Sahlin, H., Yahyapour, N. (2001) The respiratory burst response of surface-adhering leukocytes, a key to tissue engineering. Colloids and surfaces B Biointerfaces 22, 87.
139. Broberg, M., Nygren, H. (2001) von Willebrand factor, a key protein in the exposure of CD 62 on platelets. Biomaterials 22, 2403.
138. Eriksson, C., Nygren, H. (2001) PMNLs in coagulating whole blood recognise hydrophilic and hydrophobic titanium surfaces by different adhesion receptors and different patterns of recepto4r expression. J. Lab. Clin Med. 137, 296..
137. Eriksson, C., Lausmaa, J., Nygren, H. (2001) Interactions between human whole blood and modified TiO2-surfaces: Influence of surface topography and oxide thickness on leukocyte adhesion and activation. Biomaterials 22, 1987.
136. Kanagaraja, S. Wennerberg, A., Eriksson, C., Nygren, H. (2001) Cellular reactions and bone apposition to titanium surfaces with different surface roughness and oxide thickness. Biomaterials 22, 1809.
135. Eriksson, C., Nygren, H. (2001) Adhesion and activation of PMN granulocytes on titanium surfaces in contact with whole blood. J. Lab. Clin. Med. 137, 56.
134. Nygren, H., Braide, M., Eriksson, C., Yahyapour, N. (2000) Surface-adhering Human Leukocytes An In vitro model for cytotoxicity testing of fluids. ATLA 28,483.
133. Nygren, H., Braide, M., Karlsson, C. (2000) Different kinetics of the respiratory burst response in granulocyets induced by serum from blood coagulated in contact with polymer materials. Biomaterials 21, 173.
132. Karlsson, C., Braide M., Nygren, H. (2000) Interactions between whole blood and foreign materials: surface-adsorbed cellulose ethers reduce granulocyte activation by inflammatory mediators. Colloids and Surfaces B: Biointerfaces 17, 95.